Opioid-induced Decreases in Rat Brain Adenosine Levels Are Reversed by Inhibiting Adenosine Deaminase

• DOI: 10.1097/aln.0b013e3181bdf894
• Publication Date: 2009-11-19T17:52:00Z
• Authors: Ariana M. Nelson; Alanna S. Battersby; Helen A. Baghdoyan; Ralph Lydic
• Keywords: EHNA; Microdialysis

Opioids disrupt sleep and adenosine promotes sleep, but no studies have characterized the effects of opioids on levels in brain regions known to regulate states arousal. Delivering pontine reticular formation (PRF) substantia innominata (SI) region basal forebrain disrupts sleep. In contrast, administering agonists PRF or SI increases These findings encouraged current study testing hypothesis that microdialysis delivery decreases SI, respectively.A probe was placed isoflurane anesthetized rats perfused with Ringer's solution (control) followed by containing morphine (0, 10, 30, 100, 300 microm), fentanyl (100 microm) deaminase inhibitor EHNA naloxone (10 microm). Additional experiments measured before during morphine, fentanyl, plus EHNA.Morphine caused a significant (P < 0.05) concentration-dependent decrease levels. The (-20%) 100 microm blocked coadministration naloxone. Fentanyl also significantly decreased (-13.3%) adenosine. were (-26.8%) (-27.4%). both prevented alone.These data support interpretation sleep-regulating may be one mechanisms which