Isoflurane Does Not Affect Brain Cell Death, Hippocampal Neurogenesis, or Long-term Neurocognitive Outcome in Aged Rats

• DOI: 10.1097/aln.0b013e3181ca33a1
• Publication Date: 2010-01-16T07:55:16Z
• Authors: Greg Stratmann; Jeffrey W. Sall; Joseph S. Bell; Rehan S. Alvi; Laura d. V. May; Ban Ku; Mitra Dowlatshahi; Ran Dai; Philip E. Bickler; Isobel Russell; Michael T. Lee; Margit W. Hrubos; Cheryl Chiu
• Keywords: NeuN; Bromodeoxyuridine

Roughly, 10% of elderly patients develop postoperative cognitive dysfunction. General anesthesia impairs spatial memory in aged rats, but the mechanism is not known. Hippocampal neurogenesis affects learning and isoflurane neonatal young adult rats. We tested hypothesis that hippocampal function rats.Isoflurane was administered to 16-month-old rats at one minimum alveolar concentration for 4 h. FluoroJade staining performed assess brain cell death 16 h after administration. Dentate gyrus progenitor proliferation assessed by bromodeoxyuridine injection days quantification bromodeoxyuridine+ cells 12 later. Neuronal differentiation studied determining colocalization with immature neuronal marker NeuroD 5 anesthesia. New survival quantifying coexpressing mature NeuN weeks Four months anesthesia, associative fear conditioning. Spatial reference acquisition retention Morris Water Maze.Cell sporadic different between groups. did detect any differences proliferation, differentiation, new survival, or tests long-term function.In does affect death, neurogenesis, neurocognitive outcome.