High Osteogenic Potential of Adipose- and Muscle-derived Mesenchymal Stem Cells in Spinal-Ossification Model Mice

• DOI: 10.1097/brs.0000000000002266
• Publication Date: 2017-06-20T18:16:20Z
• Authors: Xizhe Liu; Gentaro Kumagai; Kanichiro Wada; Toshihiro Tanaka; Toru Asari; Kazuki Oishi; Taku Fujita; Hiroki Mizukami; Ken-Ichi Furukawa; Yasuyuki Ishibashi
• Keywords: Male; 0301 basic medicine; 0303 health sciences; Gene Expression; Cell Differentiation; Mesenchymal Stem Cells; X-Ray Microtomography; Ossification of Posterior Longitudinal Ligament; 3. Good health; Disease Models, Animal; Mice; 03 medical and health sciences; Basic Science; Adipose Tissue; Osteogenesis; Animals; Muscle, Skeletal; Spinal Cord Compression

Basic experiments in a mouse model of ossification the posterior longitudinal ligament (OPLL).To assess osteogenic potential mesenchymal stem cells (MSCs) obtained from muscle and adipose tissue Tiptoe-walking (ttw) mice, which cervical OPLL compresses spinal cord causes motor sensory dysfunction.In humans, MSCs have been implicated pathogenesis OPLL. Cervical ttw mice chronic compression cord. Few studies compared MSC with behavioral changes an animal model.We characteristics (4 to 20 weeks old) those control wild-type (without hyperostosis). Ligament was monitored by micro-computed tomography pathology; tissues were double stained fluorescent antibodies against markers for (CD45 CD105), at 8 weeks. The Basso Mouse Scale used function, heat mechanical tests function. assessed Alizarin Red S absorbance, staining mineralization, real-time quantitative polymerase chain reaction osteogenesis-related genes.Spinal-ligament began age, ossified area increased age. Immunofluorescence identified area. became hyposensitive scores showed motor-function deficits starting 12 mineralization higher adipose- muscle-derived than 4, 8, Real-time that strongly expressed genes.MSCs derived had high potential.N/A.