Real-Time Direct Measurement of Spinal Cord Blood Flow at the Site of Compression

• DOI: 10.1097/brs.0b013e3181316310
• Publication Date: 2007-08-10T07:07:57Z
• Authors: Yuichiro Hamamoto; Tadanori Ogata; Tadao Morino; Masayuki Hino; Haruyasu Yamamoto
• Keywords: Spinal cord compression

An in vivo study to measure rat spinal cord blood flow real-time at the site of compression using a newly developed device.To evaluate change thoracic by force and clarify association between recovery motor deficiency after injury.Until now, no measurement has been conducted. In addition, it not clearly determined whether is related function injury.Our system was combination noncontact type laser Doppler device. The exposed 11th vertebra continuously measured before, during, compression. functioning animal's hind-limbs evaluated Basso, Beattie Bresnahan scoring scale frequency voluntary standing. Histologic changes such as permeability blood-spinal barrier, microglia proliferation, apoptotic cell death were examined compressed tissue.The decreased on each increase force. After applying 5-g weight, <40% precompression level. Complete ischemia reached 20-g weight. decompression, level 20-minute complete group significantly higher than that 40-minute group. hind-limb less sham (without compression), while significant difference observed group, rats whose incomplete showed loss compared with completely recovered flow. Extensive breakdown barrier integrity following proliferation detected group.Duration ischemia/compression are important factors injury.