Comparative proteomic analysis of irinotecan-sensitive colorectal carcinoma cell line and its chemoresistant counterpart

• DOI: 10.1097/cad.0b013e3283408596
• Publication Date: 2011-03-25T20:11:16Z
• Authors: Feng-Ming Gong; Xing-Chen Peng; Ben-Xu Tan; Jun Ge; Xi Chen; Ye Chen; Feng Xu; Feng Bi; Jian-Mei Hou; Ji-Yan Liu
• Keywords: Proteomics; 0301 basic medicine; 0303 health sciences; Cell Survival; Blotting, Western; Molecular Sequence Data; Irinotecan; Antineoplastic Agents, Phytogenic; Neoplasm Proteins; 3. Good health; 03 medical and health sciences; Actin Depolymerizing Factors; Drug Resistance, Neoplasm; Tandem Mass Spectrometry; Cell Line, Tumor; Phosphopyruvate Hydratase; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Image Processing, Computer-Assisted; Humans; Camptothecin; Electrophoresis, Gel, Two-Dimensional; Amino Acid Sequence; Colorectal Neoplasms

In this study, we used two-dimensional gel electrophoresis and MALDI-Q-TOF-MS/MS analysis to examine the global protein expression of a pair colorectal carcinoma cell lines, SW620 irinotecan-resistant SW620. Of 30 spots identified as differentially expressed proteins (±over twofold, P<0.05) between two 26 (corresponding unique proteins) were positively by analysis. These could be grouped into main classes including metabolism (15.38%), SSproliferation/differentiation (11.53%), molecular chaperone mRNA splicing so on. The proteins, which might involved in development tumor drug resistance, such α-enolase, cofilin, thioredoxin-dependent peroxide 1, have been validated western blot discussed. study may useful showing mechanisms underlying irinotecan resistance.