Effective anti-tumor responses induced by recombinant bacillus Calmette–Guérin vaccines based on different tandem repeats of MUC1 and GM-CSF

• DOI: 10.1097/cej.0b013e32832c3882
• Publication Date: 2009-08-03T09:08:13Z
• Authors: Shifang Yuan; Changhong Shi; Wei Han; Rui Ling; Nanlin Li; Ting Wang
• Keywords: MUC1

In this study, we constructed several novel breast cancer vaccines, Bacillus Calmette-Guérin (BCG)-MUC1 variable-number tandem repeats (VNTR) 1/4/8-CSF, that consist of BCG and express 1, 4, 8 the MUC1 human granulocyte-macrophage colony-stimulating factor (GM-CSF). The ability three recombinant vaccines to inhibit growth was observed in (hu)-peripheral blood lymphocyte (PBL)-severe combined immunodeficient (SCID) mice. Prophylactic protective responses were successfully induced tumor incidence mice immunized with (rBCG)-MVNTR4-CSF (37.5%) or rBCG-MVNTR8-CSF (25%) significantly decreased compared control (100%) at 42 days after implantation (P<0.05 vs. group). We also found CD4-positive CD8-positive lymphocytes detected only tumors grown rBCG-MVNTR4/8-CSF-immunized animals, strong IFN-gamma by immunization rBCG-MVNTR4-CSF vaccines. This study suggests a potential role coexpressed GM-CSF eliciting tumor-specific immune response. Our results indicate preventively inhibited hu-PBL-SCID both rBCG may be good candidates for immunotherapy.