Oral Pretreatment With Liposomal Glutathione Attenuates Reperfusion Injury in Rabbit Isolated Hearts

• DOI: 10.1097/fjc.0b013e31827c0f02
• Publication Date: 2012-11-22T07:41:42Z
• Authors: D. Adam Lauver; Nayiri M. Kaissarian; Benedict R. Lucchesi
• Keywords: Male; 0301 basic medicine; Cardiotonic Agents; Time Factors; Myocardium; Myocardial Ischemia; Myocardial Reperfusion Injury; In Vitro Techniques; Glutathione; Myocardial Contraction; Antioxidants; 3. Good health; Perfusion; Oxidative Stress; Random Allocation; 03 medical and health sciences; Intestinal Absorption; Malondialdehyde; Dietary Supplements; Liposomes; Animals; Lipid Peroxidation; Rabbits

Reactive oxygen species are a key mediator of myocardial reperfusion injury. Endogenous cellular defenses against reactive often become overwhelmed after ischemia and reperfusion. Therefore, exogenous supplementation various antioxidant compounds has been hypothesized to protect Reduced glutathione (GSH) is an important endogenous that affords protection oxidative damage. Oral administration GSH limited due poor gastrointestinal absorption. A liposomal preparation (lipGSH) capable oral was investigated for its ability attenuate tissue injury increase levels in isolated heart model Male, New Zealand white rabbits were assigned randomly among 4 groups as follows: control daily lipGSH 3, 7, or 14 days. At completion the dosing regimen, hearts harvested perfused retrograde manner with use Langendorff apparatus. The subjected 30 minutes global followed by 60 Hearts from lipGSH-treated exhibited better recovery left ventricular contractile function during had attenuated Furthermore, animals increased demonstrating effective absorption lipGSH.