Determination of Linezolid in Human Plasma by High-Performance Liquid Chromatography With Ultraviolet Detection

• DOI: 10.1097/ftd.0b013e3181d5eeee
• Publication Date: 2010-03-18T09:34:48Z
• Authors: Dario Cattaneo; Sara Baldelli; Francesca Conti; Valeria Cozzi; Emilio Clementi
• Keywords: Protein precipitation; Phosphoric acid; Linezolid; Perchloric acid; Therapeutic Drug Monitoring

A high-performance liquid chromatographic method for the determination of linezolid in human plasma was developed and validated. After precipitation proteins with perchloric acid, protein-free supernatant separated by isocratic reverse-phase chromatography on a X Bridge C18 column. The mobile phase consisted mixture phosphoric acid 0.05%: acetonitrile (75:25, v/v) flow rate 1 mL/min. column elute monitored at 254 nm. linear from 0.2 to 48 mg/L (mean r2 = 0.9996, n 10). observed intra- inter-day assay imprecision ranged 2.83% 8.16% (18.80% lower limit quantification); inaccuracy varied between -0.33% 8.18%. Mean drug recovery 99.8% 90.0% internal standard (para-toluic acid). found be precise accurate suitable therapeutic monitoring routine clinical practice.