IN VIVO SAFETY OF TRYPAN BLUE USE IN VITREORETINAL SURGERY

• DOI: 10.1097/iae.0b013e3182003adb
• Publication Date: 2010-12-23T05:44:20Z
• Authors: EHAB A ABDELKADER; VIKKI A McBAIN; MRINAL ANAND; NEIL W SCOTT; M A REHMAN SIDDIQUI; NOEMI LOIS
• Keywords: Male; Intraoperative Care; Visual Acuity; 610; Epiretinal Membrane; Trypan Blue; Middle Aged; Vitreoretinal Surgery; Retinal Perforations; Basement Membrane; Fluorescence; Retina; 03 medical and health sciences; 0302 clinical medicine; 616; Electroretinography; Humans; Female; Prospective Studies; Coloring Agents; Aged

The purpose of this study was to evaluate "in vivo" safety of trypan blue (TB) in patients undergoing TB-assisted internal limiting membrane or epiretinal membrane peeling.Prospective study including 21 patients (21 eyes) with full-thickness macular hole and/or epiretinal membrane undergoing TB-assisted internal limiting membrane/epiretinal membrane peeling. Main outcome measures included distance visual acuity, near visual acuity, amplitude of P50 and N95 of the pattern electroretinogram, and fundus autofluorescence; these were assessed preoperatively, at 6 months (n = 21) and 12 months (n = 10) postoperatively.There was a statistically significant improvement in distance visual acuity, near visual acuity, P50, and N95 amplitude at 6 months and 12 months postoperatively. The mean logarithm of the minimum angle of resolution distance visual acuity and near visual acuity improved from baseline by 0.31 (SD 0.37) and 0.17 (SD 0.31) at 6 months, respectively, and by 0.4 (SD 0.25) and 0.35 (SD 0.28) at 12 months, respectively. The mean P50 and N95 component amplitudes improved by 28% compared with baseline at 6 months (P50 0.4 [SD 0.8]; N95 0.53 [SD 1.07]) and by 63% at 12 months (P50 0.9 [0.85]; N95 1.04 [1.34]). Autofluorescence did not demonstrate damage to the retinal pigment epithelium attributable to TB.No deleterious effects of TB were observed in this study.