Glucocorticoids Suppress Corneal Lymphangiogenesis

• DOI: 10.1097/ico.0b013e318213f39f
• Publication Date: 2011-10-05T00:01:48Z
• Authors: Maria M Steele; Philip M Kelley; Amanda M Schieler; Richard M Tempero
• Keywords: Lymphangiogenesis; Lymphatic Endothelium; Lymphatic vessel

Purpose: To determine whether glucocorticoids suppress corneal lymphatic vessel growth (lymphangiogenesis) or induce regression. Methods: We measured human endothelial cell proliferation and collagen-induced tubulogenesis in culture conditions with without dexamethasone, a potent glucocorticoid. developed modification of the mouse suture model that allowed us to visualize (with suture) regression (suture removed) using immunofluorescence microscopic techniques. administered dexamethasone vehicle control mice sutured corneas. visualized quantified vessels. vascular factor-C tumor necrosis factor-α messenger RNA expression unsutured corneas quantitative reverse transcriptase–polymerase chain reaction. Results: High-dose did not change properties cells vitro. demonstrated suppressed rather than treated dexamethasone. Expressions were similar Conclusions: Dexamethasone new These findings identify novel mechanism glucocorticoid action: suppression lymphangiogenesis.