Effects and Mechanisms of Anti-CD44 Monoclonal Antibody A3D8 on Proliferation and Apoptosis of Sphere-Forming Cells With Stemness From Human Ovarian Cancer

• DOI: 10.1097/igc.0b013e3182a1d023
• Publication Date: 2013-09-24T12:08:29Z
• Authors: Yong-Rui Du; Ying Chen; Yan Gao; Xiu-Long Niu; Ya-Jing Li; Wei-Min Deng
• Keywords: Cyclin-Dependent Kinase Inhibitor p21; Ovarian Neoplasms; 0301 basic medicine; Caspase 3; Cyclin-Dependent Kinase 2; Antibodies, Monoclonal; Ascites; Apoptosis; Cyclin A; Adenocarcinoma; 3. Good health; 03 medical and health sciences; Hyaluronan Receptors; Gene Expression Regulation; Proto-Oncogene Proteins c-bcl-2; Cell Line, Tumor; Neoplastic Stem Cells; Humans; Female; Cisplatin; Cell Proliferation

CD44(+) human ovarian cancer stem cells (CSCs) and CSC-like have been identified characterized. Compelling evidence has revealed that CD44 is involved in the occurrence development of cancers. Our previous study showed sphere-forming (SFCs) from cell line SKOV-3 had CSC capacity. Therefore, present study, we aimed to investigate effects mechanisms anti-CD44 monoclonal antibody A3D8 on proliferation apoptosis SFCs explore novel strategies for treatment cancer.We investigated using MTS assay, cycle analysis, an annexin V-fluorescein isothiocyanate/propidium iodide kit, Rh123 detection real-time reverse transcription polymerase chain reaction Western blotting.After ligation by A3D8, SFC was notably attenuated, progression arrested S phase, significantly increased. The effect enhanced a dose- time-dependent manner, induction DDP combination with A3D8. Furthermore, messenger RNA expression levels p21 caspase-3 were up-regulated, whereas those CDK2, cyclinA, Bcl-2 down-regulated. protein down-regulated.Our findings indicate antibodies may be potential strategy after conventional therapy via inhibition growth promotion stemness.