Persistence of Adenovirus Nucleic Acids in Nasopharyngeal Secretions

• DOI: 10.1097/inf.0b013e3181d743c8
• Publication Date: 2010-03-23T06:43:35Z
• Authors: Stella U. Kalu; Michael Loeffelholz; Eric Beck; Janak A. Patel; Krystal Revai; Jiang Fan; Kelly J. Henrickson; Tasnee Chonmaitree
• Keywords: Adenovirus infection; Persistence (discontinuity)

Background: Polymerase chain reaction (PCR) assays increase the rate of viral detection in clinical specimens, compared with conventional virologic methods. Studies suggest that PCR may detect virus nucleic acid (NA) persists respiratory tract. Methods: We analyzed data from children having frequent upper infections (URI), who were followed up a longitudinal study. Nasopharyngeal secretions collected at URI onset and when acute otitis media was diagnosed; studies performed using diagnostics PCR. Repeated presence adenovirus by further studied sequencing phylogenetic analysis. Results: Of 581 episodes 76 children, 510 viruses detected. detected PCR, 15% those previously; repeated positives occurred most frequently adenovirus. Sequencing results available 13 detection; following 4 patterns infection identified (16 instances): (1) same serotype strain continuously (n = 8 instances), (2) different serotypes during sequential 3), (3) but strains (4) intermittently 2). Conclusions: Among URIs, positive for NA represent new serotype/strain, or persistence NA. Results must be interpreted caution; correlation other are important. Further after required better understanding significance tests