Genetic Relatedness of Coagulase-negative Staphylococci From Gastrointestinal Tract and Blood of Preterm Neonates With Late-onset Sepsis

• DOI: 10.1097/inf.0b013e3182791abd
• Publication Date: 2012-10-18T19:10:46Z
• Authors: Hiie Soeorg; Kristi Huik; Ülle Parm; Mari-Liis Ilmoja; Natalja Metelskaja; Tuuli Metsvaht; Irja Lutsar
• Keywords: Staphylococcus haemolyticus; cons; Coagulase; Bacteremia; Multilocus sequence typing; Blood Culture

Coagulase-negative staphylococci (CoNS) are the first colonizers of gastrointestinal tract (GIT) and commonest cause late-onset sepsis (LOS) in preterm neonates. Intravascular catheters considered a major source CoNS bacteremia. However, several cases LOS remain without an identified source. To elucidate whether GIT could be potential invasive strains, we aimed to assess molecular similarity between from blood neonates with LOS.Altogether 22 53 isolates collected caused by (Staphylococcus haemolyticus 13, Staphylococcus epidermidis 7 hominis 2 patients) were included. Rectal swabs twice weekly birth, but only obtained before analyzed. S. typed multilocus variable number tandem repeats analysis sequence typing, pulsed-field gel electrophoresis.Eighteen had same species bloodstream; all these them (altogether 18 28 isolates) underwent typing. The genotypic bloodstream ≥1 antecedent was observed 13 patients-3 10 11 infection. concordant 0-7 days positive culture.The indicates that harbour strains LOS. Whether there is causal relationship colonization remains elucidated further studies.