Silencing of the Candidate Tumor Suppressor Gene Solute Carrier Family 5 Member 8 (SLC5A8) in Human Pancreatic Cancer

• DOI: 10.1097/mpa.0b013e3181630ffe
• Publication Date: 2008-04-21T07:04:05Z
• Authors: Jong Y. Park; James F. Helm; Weipeng Zheng; Quan P. Ly; Pamela J. Hodul; Barbara A. Centeno; Mokenge P. Malafa
• Keywords: Trichostatin A; Bisulfite sequencing; Cancer Epigenetics

Few genetic mutations have been identified in pancreatic adenocarcinoma, whereas epigenetic changes that lead to gene silencing are known several genes. Because SLC5A8 is regarded as a potential tumor suppressor down-regulated by other cancers, we sought characterize promoter methylation status and its relationship expression cancer.Promoter of were evaluated cancer cell lines, tumor, adjacent nontumor tissues from patients using methylation-specific polymerase chain reaction analysis, quantitative real-time semiquantitative reverse transcriptase-polymerase reaction, bisulfate-modified sequencing.Complete or partial loss was observed all tissues. Bisulfite sequencing analysis on lines did not express detected dense the region. reactivated treatment with aza-deoxycytidine trichostatin A. Methylation-specific 7 10 tissues, only 3 28 (P < 0.001).Our findings indicate result aberrant adenocarcinoma. We suggest may function whose contribute carcinogenesis progression cancer.