Clinical Validation of a Real-Time Polymerase Chain Reaction Detection of Neisseria gonorrheae porA Pseudogene Versus Culture Techniques

• DOI: 10.1097/olq.0b013e3181644bc9
• Publication Date: 2009-03-04T18:48:25Z
• Authors: Stig Ove Hjelmevoll; Merethe Elise Olsen; Johanna U.Ericson...; Håkon Haaheim; Kjetil K. Melby; Harald Moi; Magnus Unemo; Vegard Skogen
• Keywords: Pseudogene; Neisseria gonorrhoeae; Neisseria

In Brief Background: Diagnosing Neisseria gonorrheae using nucleic acid amplification tests (NAATs) might increase the sensitivity, compared to cultivation. However, NAATs has also been problematic mainly due close genetic relationships between different species, resulting in false positive diagnoses. This study was conducted clinically validate a previously published real-time polymerase chain reaction (PCR) method targeting porA pseudogene N. comparison culture techniques. Methods: total, 360 samples, urethra (n = 109), rectum 84), pharynx 119), and cervix 48) from 185 males 57 females, were analyzed PCR Sequencing of entire 16S rRNA gene used resolve discrepant results. Results: Of 37 by both PCR, however, identified 15 additional confirmed samples. The showed specificity, predictive value, negative value 100% preselected population. population had true gonorrhea prevalence 17.4%. Conclusions: present comprises valuable supplement traditional techniques for diagnosis gonorrheae, especially samples extragenital sites such as rectum. A performance diagnostic detecting gonorrhoeae genital well extra with excellent sensitivity specificity.