The Application of Mitochondrial DNA Cytochrome Oxidase II Gene for the Identification of Forensically Important Blowflies in Western China

• DOI: 10.1097/paf.0b013e31815b4889
• Publication Date: 2009-03-05T02:16:36Z
• Authors: Bin Wu Ying; Ting Ting Liu; Hong Fan; Dong Wei; Fu Qiang Wen; Peng Bai; Jin Huang; Yi Ping Hou
• Keywords: Lucilia cuprina; Lucilia; Sister group

Blowflies found on human corpses are important for the estimation of postmortem interval and other questions forensic relevance. Some these species difficult to differentiate morphologically, therefore a molecular method was elaborated identification. Here, we describe rapid identification insects. Specific insect DNA fragments were amplified using polymerase chain reaction, followed by direct sequencing amplification products. Analysis cytochrome oxidase II sequences revealed abundant phylogenetically informative nucleotide substitutions that could identify blowfly group. In contrast, because low level sequence divergence sister species, data not distinguish among taxa from same group, ie, within Lucilia sericata cuprina groups. The support existing group separation Calliphora. Because speed accuracy current technology apomorphic available mtDNA sequences, this approach enables quick used interval.