Adipogenic Potential of Adipose Stem Cell Subpopulations

• DOI: 10.1097/prs.0b013e318221db33
• Publication Date: 2011-05-13T09:33:28Z
• Authors: Han Li; Ludovic Zimmerlin; Kacey G. Marra; Vera S. Donnenberg; Albert D. Donnenberg; J. Peter Rubin
• Keywords: Stromal vascular fraction; CD31; CD146

Adipose stem cells represent a heterogenous population. Understanding the functional characteristics of subpopulations will be useful in developing adipose cell-based therapies for regenerative medicine applications. The aim this study was to define distinct populations within stromal vascular fraction based on surface marker expression, and evaluate ability each cell type differentiate mature adipocytes.Subcutaneous whole tissue obtained by abdominoplasty from human patients. separated four were isolated flow cytometry studied. Candidate perivascular (pericytes) defined as CD146(+)/CD31(-)/CD34(-). Two CD31(+) endothelial detected differentiated CD34 expression. These tentatively designated (CD31(+)/CD34(-)), immature (CD31(+)/CD34(+)). Both heterogeneous with respect CD146. CD31(-)/CD34(+) (preadipocyte candidate) also CD90(+) but lacked CD146 expression.Proliferation greatest group slowest group. Expression adipogenic genes, peroxisome proliferator-activated receptor-γ, fatty acid binding protein 4, significantly higher compared all other after vitro differentiation. This demonstrated highest proportion AdipoRed lipid staining.The authors have adult characterized their potential. Of these populations, CD31/CD34(+) is most prevalent has potential appears hold promise engineering.