The Effect of Age on Human Adipose-Derived Stem Cells

• DOI: 10.1097/prs.0b013e3182729cfc
• Publication Date: 2012-11-17T08:17:53Z
• Authors: Wei Wu; Laura Niklason; Derek M. Steinbacher
• Keywords: Adult; Aged, 80 and over; Aging; 0303 health sciences; Osteocalcin; Infant; Neovascularization, Physiologic; Core Binding Factor Alpha 1 Subunit; Mesenchymal Stem Cells; Middle Aged; Telomere; Flow Cytometry; Real-Time Polymerase Chain Reaction; Subcutaneous Fat, Abdominal; 03 medical and health sciences; Osteogenesis; Humans; Intercellular Signaling Peptides and Proteins; Biomarkers; Cells, Cultured; Cellular Senescence; Aged

Adipose-derived mesenchymal stem cells are a robust, multipotent cell source. They easily harvested and exhibit promise in variety of regenerative applications. The purpose this study was to evaluate the aging impact on adipose-derived cells, relating morphology, senescent properties, growth factor expression, osteogenesis.Cells obtained from distinct age groups (infant, adult, elderly) were cultured. Morphology examined using microscopy, surface markers interrogated flow cytometry. Telomere length measured real-time polymerase chain reaction. Expression pertinent angiogenic osteogenic factors compared. Osteogenic capability investigated further by evaluating induction response, quantification mRNA expression RUNX-2 osteocalcin.The same isolating ratio derived each donor, regardless age. infant exhibited elongated spindle morphology increased telomere compared with older cells. Angiogenic more highly expressed whereas similar among all ages. Response profound than as evidenced alkaline phosphatase alizarin red staining, bone-related gene expression.Adipose-derived available across groups. Infant-derived morphologically spindle-shaped, long telomeres, enhanced capabilities Conversely, paracrine activity, authors posit that clinical applicability is conserved during adult elderly period.