HIV-1 Genetic Diversity and Transmitted Drug Resistance in Health Care Settings in Maputo, Mozambique

• DOI: 10.1097/qai.0b013e3181a24906
• Publication Date: 2009-06-22T09:07:16Z
• Authors: Inês Bártolo; José Casanovas; Rui Bastos; Cheila Rocha; Ana B Abecasis; Elena Folgosa; José Mondlane; Rolanda Manuel; Nuno Taveira
• Keywords: Subtyping; Reverse-transcriptase inhibitor

To characterize HIV-1 diversity and transmitted drug resistance in persons with access to care treatment Maputo, Mozambique.Samples were collected 2002-2004 from 144 drug-naive patients attending public hospitals private clinics. Plasma viremia, CD4, CD8 cell counts determined for each patient. The Stanford Algorithm was used genotyping on pol sequences. Subtyping done by phylogenetic analysis.Most had high viral load (mean, 5.0 log copies/mL) low CD4 (median, 260 cells/microL). Protease and/or reverse transcriptase sequences obtained 104 (72%) samples. Patients harbored subtypes C (80.8%), G (3.8%), CRF37_cpx (6.7%), untypable (U) (1.0%), recombinant strains (7.7%) comprising the A, C, D, F, U clades. There no major protease inhibitor mutations. Mutations conferring nucleoside/nucleotide inhibitors nonnucleoside found 4 (4/68; 5.9%) patients. Phylogenetic analysis suggested an imported origin 2 resistant variants.The epidemic Maputo is evolving rapidly genetic complexity due recent introduction of all forms. Continued surveillance treated untreated populations needed prevent further transmission HIV drug-resistant variants maximize efficacy antiretroviral therapy Maputo.