Elevated Natural Killer Cell Activity Despite Altered Functional and Phenotypic Profile in Ugandans With HIV-1 Clade A or Clade D Infection

• DOI: 10.1097/qai.0b013e3181aa256e
• Publication Date: 2009-07-06T07:29:49Z
• Authors: Michael A Eller; Leigh Anne Eller; Benson J Ouma; Doris Thelian; Veronica D Gonzalez; David Guwatudde; Francine E McCutchan; Mary A Marovich; Nelson L Michael; Mark S de Souza; Fred Wabwire-Mangen; Merlin L Robb; Jeffrey R Currier; Johan K Sandberg
• Keywords: Adult; Male; 0301 basic medicine; Acquired Immunodeficiency Syndrome; Middle Aged; CD56 Antigen; CD4 Lymphocyte Count; Immunophenotyping; 3. Good health; Killer Cells, Natural; Interferon-gamma; 03 medical and health sciences; Lysosomal-Associated Membrane Protein 1; Receptors, KIR2DL1; HIV-1; Humans; Female; Uganda; NK Cell Lectin-Like Receptor Subfamily C

Background and Objective: Natural killer (NK) cells most likely contribute toward limiting HIV-1 replication, investigation into their function throughout the course of infection is therefore important. We here aimed to determine state NK cell compartment in Ugandans with untreated clade A or D comparison matched uninfected controls. Methods Results: The phenotype were investigated using 10-color flow cytometry. Surprisingly, displayed elevated production interferon-γ macrophage inflammatory protein 1β, as well CD107a degranulation infected subjects. This included unexpected levels CD56bright subset high 1β CD56negative cells. was associated reduced expression KIR2DL1, NKG2A, CD161, NKp30 CD56dim cells, whereas lowered CD161 only alteration subset. Interestingly, low CD4 counts increased NKp44 Conclusions: HIV-1-infected display activity, despite an altered functional phenotypic profile. Furthermore, specific alterations subsets occur patients severe loss.