Assessment of Intra-arterial Injected Autologous Bone Marrow Mononuclear Cell Distribution by Radioactive Labeling in Acute Ischemic Stroke

• DOI: 10.1097/rlu.0b013e318156b980
• Publication Date: 2009-03-03T23:01:48Z
• Authors: Patricia Lavatori...; Claudio T. Mesquita; Renata M. Felix; Jader C. Azevedo; Gustavo B. Barbirato; Carlos Henrique F...; Constantino Gonzalez; Maria Lucia Mendonça; Aquiles Manfrim; Gabriel de Freitas; Cristina C. Oliveira; Daniel Silva; Dione Ávila; Radovan Borojevic; Suzana Alves; Amarino C. Oliveira; Hans F. Dohmann
• Keywords: Stroke

Objective: To evaluate the feasibility of monitoring autologous mononuclear bone marrow (ABMMN) cells implanted into brain after acute ischemic stroke by technique labeling with Tc-99m-HMPAO. Case Report: A 37-year-old man presented aphasia, right-side hypoesthesia, and right homonymous hemianopsia an left middle cerebral artery. He was included in cell-based therapy research protocol about safety intra-arterial cell transplantation for stroke. Nine days he received 3.0 × 107 ABMMN delivered artery via a balloon catheter. Approximately 1% these were labeled 150 MBq (4 mCi) Tc-99m incubation hexamethylpropylene amine oxime (HMPAO). Results: Brain perfusion images ECD demonstrated hypoperfusion temporal parietal regions. The compared tomographic views obtained 8 hours ABMMN-labeled delivery, revealing intense accumulation ipsilateral hemisphere. whole-body scan done showed brain, liver, spleen uptake. Conclusions: Our results that HMPAO can be used to label vivo visualization, SPECT imaging is feasible noninvasive method studying fate transplanted vivo. Additionally, our findings demonstrate localization intra-arterially injected cells.