Influence of Human Adipose-Derived Stromal Cells on Wnt Signaling in Organotypic Skin Culture

• DOI: 10.1097/scs.0b013e3182077fa2
• Publication Date: 2011-03-17T08:27:30Z
• Authors: Deok-Woo Kim; Jae-Sun Lee; Eul-Sik Yoon; Byung-Il Lee; Seung-Ha Park; Eun-Sang Dhong
• Keywords: Human skin; Skin repair; DKK1

Background: Human adipose-derived stromal cells (hASCs) produce various cytokines. Also, there is a growing opinion that large proportion of the useful effects cell therapy may be attributable to secretion Several reports suggested beneficial hASCs on skin. These include antioxidant activity, accelerated wound healing, whitening effects, and antiaging. We investigated effect skin Wnt signaling, which associated with regeneration differentiation. Methods: Pieces human were cocultured hASCs, 2 chambered transwell culture plates used prevent direct contact between In control group, pieces cultured without hASCs. Wnt1, Axin2, TCF1, LEF1, DKK1 mRNA expressions quantitatively assessed using real-time polymerase chain reaction. The expression levels β-catenin compared Western blot immunohistochemical analyses. Results: Wnt1 LEF1 was positively influenced by presence in medium (P < 0.05). total protein level hASC-cocultured higher than group. Immunohistochemical staining showed β-catenin-stained area dermis larger group most stained fibroblasts. Conclusions: results current study promoted canonical signaling organotypic through paracrine increased mainly due dermal