THERMAL INJURY ELEVATES THE INFLAMMATORY MONOCYTE SUBPOPULATION IN MULTIPLE COMPARTMENTS

• DOI: 10.1097/shk.0b013e31805362ed
• Publication Date: 2007-07-06T08:21:34Z
• Authors: John G. Noel; Andrew Osterburg; Quan Wang; Xialing Guo; Denise Byrum; Sandy Schwemberger; Holly Goetzman; Charles C. Caldwell; Cora K. Ogle
• Keywords: Monocyte

Recent publications have demonstrated that human resident and inflammatory monocyte (IM) subpopulations equivalents in rodents. The effect of thermal injury upon these has not been studied. Mice were given a scald burn killed on postburn days (PBDs) 2, 4, 8. Bone marrow, blood, spleen white cells isolated, the percentage monocytes (CD11b LY6C), IMs progenitors (macrophage-colony-forming unit [M-CFU]) determined. ability each population to make TNF-alpha was determined by intracellular cytokine staining. Finally, sorted fractions from PBD 8 inhibit lymphocyte proliferation performed. We noted there an increase M-CFU blood at 8, but marrow only had nonsignificant M-CFU. All compartments showed significant number no changes seen. In all compartments, major source TNF-alpha. accompanied chemokine chemoattractant protein 1 constitutively high levels progenitor stromal-derived factor 1alpha. After injury, mice deficient receptor for soluble equal splenic monocytes, as did wild-type mice, suggesting this is essential effect. conclude model, are vivo