CD4+CD25+ Regulatory T Cells Attenuate Lipopolysaccharide-Induced Systemic Inflammatory Responses and Promotes Survival in Murine Escherichia coli Infection

• DOI: 10.1097/shk.0b013e318296e65b
• Publication Date: 2013-05-02T01:23:29Z
• Authors: Emeka B. Okeke; Ifeoma Okwor; Zhirong Mou; Ping Jia; Jude E. Uzonna
• Keywords: Proinflammatory cytokine; Adoptive Cell Transfer

It is well established that CD4CD25 regulatory T cells (Tregs) downregulate inflammatory immune responses and help to maintain homeostasis. Recent reports have shown ligation of germline encoded pattern recognition receptors such as Toll-like can stimulate Tregs therefore implicate in the pathophysiology sepsis other diseases. In this report, we show injection lipopolysaccharide (LPS) leads expansion CD4CD25FoxP3 Tregs, suggesting these may play an important role regulation LPS-induced acute inflammation. Indeed, genetic or immunological inhibition Treg function using mice lacking functional (CD25 KO mice) anti-CD25 monoclonal antibody (anti-CD25 mAb), respectively, led death otherwise nonlethal LPS challenge. This was accompanied by exaggerated production proinflammatory cytokines. Strikingly, adoptive transfer CD25 before challenge rescues from death. Unlike LPS, depletion followed concanavalin A (Con A) does not result mortality, globally influence all models We authenticate our findings showing mortality a Escherichia coli elevated serum levels Collectively, results indicate addition inflammation, improve bacterial clearance promote survival model infection.