Protective Effects of Fresh Frozen Plasma on Vascular Endothelial Permeability, Coagulation, and Resuscitation After Hemorrhagic Shock Are Time Dependent and Diminish Between Days 0 and 5 After Thaw

• DOI: 10.1097/ta.0b013e3181e453d4
• Publication Date: 2010-07-12T10:34:55Z
• Authors: Shibani Pati; Nena Matijevic; Marie-Françoise D...; Tien Ko; Yanna Cao; Xiyun Deng; Rosemary A. Kozar; Elizabeth Hartwell; Jodie Conyers; John B. Holcomb
• Keywords: Fresh frozen plasma; Vascular permeability

Background: Clinical studies have shown that resuscitation with fresh frozen plasma (FFP) is associated improved outcome after severe hemorrhagic shock (HS). We hypothesized in addition to its effects on hemostasis, FFP has protective and stabilizing the endothelium translate into diminished endothelial cell (EC) permeability vivo HS. further beneficial of would diminish over 5 days routine storage at 4°C. Methods: EC was induced by hypoxia assessed passage 70-kDa Dextran between monolayers. Thrombin generation time coagulation factor levels or activity were FFP. An rat model HS used determine hemodynamic stability. Results: Thawed inhibits vitro 10.2-fold. Protective (to 2.5-fold) day 5. increased been stored 0 In data show superior maintaining mean arterial pressure rats undergoing resuscitation. Conclusion: Both permeability, vascular stability, The benefits are independent hemostasis storage.