Erythropoietin Administration Modulates Pulmonary Nrf2 Signaling Pathway After Traumatic Brain Injury in Mice

• DOI: 10.1097/ta.0b013e3181f6b984
• Publication Date: 2011-03-10T08:15:07Z
• Authors: Wei Jin; Jun Wu; Handong Wang; Jie Kong; Hongbin Ni; Weibang Liang
• Keywords: Vascular permeability

In our previous studies, antioxidant transcription factor, nuclear factor erythroid 2-related 2 (Nrf2) signaling pathway has been shown to play an important role in protecting traumatic brain injury (TBI)-induced acute lung (ALI). This study was designed explore whether recombinant human erythropoietin (rhEPO) administration modulates pulmonary Nrf2 a murine TBI model.Closed head made by Hall's weight-dropping method. The rhEPO administered at dose of 5,000 IU/kg 30 minutes after TBI. Pulmonary capillary permeability, wet or dry weight ratio, apoptosis, and its downstream cytoprotective enzymes including NAD(P)H:quinone oxidoreductase 1, glutathione S-transferase were investigated 24 hours TBI.We found that treatment with markedly ameliorated TBI-induced ALI, as characterized decreased alveolar cells apoptosis. Administration also significantly upregulated the mRNA expressions activities pathway-related agents, Nrf2, S-transferase.The results this suggest post-TBI may induce Nrf2-mediated response lung, be mechanism whereby reduces ALI.