Preservation of Splenic Immunocompetence After Splenic Artery Angioembolization for Blunt Splenic Injury

• DOI: 10.1097/ta.0b013e3181f9fa1e
• Publication Date: 2010-11-11T07:13:34Z
• Authors: Ajai K. Malhotra; Richard F. Carter; Deborah A. Lebman; Dawn S. Carter; Omer J. Riaz; Michel B. Aboutanos; Therese M. Duane; Rao R. Ivatury
• Keywords: Immunocompetence

Background: Splenic artery angioembolization (SAE) is increasingly being used as an adjunct to nonoperative management for stable patients with blunt splenic injury (BSI). However, little known about immunocompetence after SAE. This study aims at assessing SAE BSI. Methods: Peripheral blood was obtained from BSI (n = 8) who had >6 months prior. assessed by isolating mononuclear cells and incubating CD4+ CD45RA+ CD45RO+ antibody analyze the proportion of T-cells expressing CD4 receptor, or coexpressing either CD45RA CD45RO receptors. Cells were counted fluorescence-activated cell sorting compared trauma that splenectomy also prior 4, negative controls) normal healthy volunteers intact spleens positive controls). Results: The test discriminatory asplenic state. %CD4+ significantly lower in splenectomized (16 ± 1) versus (40 2), p < 0.05. due significant decrease (8 2 vs. 26 0.05) %CD4+CD45RA+ whereas CD4+CD45RO+ maintained similar normal. values (CD4+, 36 2, CD4+CD45RA+, 24 2) comparable (p > higher than 0.05). When group subdivided into main (total, n 4) branch vessel (partial, SAE, results same both types Conclusion: immune function, measured T-cell subset, generated only presence immunocompetent spleen, preserved BSI, partial.