Identification of an Expressed Truncated Form of CD200, CD200tr, which is a Physiologic Antagonist of CD200-Induced Suppression

• DOI: 10.1097/tp.0b013e318186fec2
• Publication Date: 2008-10-17T07:09:13Z
• Authors: Zhiqi Chen; Dang-Xiao Chen; Yu Kai; Ismat Khatri; Brent Lamptey; Reginald M. Gorcz...
• Keywords: Mixed lymphocyte reaction

Earlier studies have indicated that cell surface expressed CD200, or a soluble form of this molecule, can induce immunosuppression in number biological systems, and promote increased graft acceptance, after binding to receptors (CD200Rs). Many groups reported the NH2-terminal region CD200 is crucial for interaction with CD200R.A truncated full-length, immunosuppression-inducing representing translation an mRNA splice variant, CD200tr, was CHO cells transduced used produce mAbs unique CD200tr. These mAbs, full-length were document physiologic expression CD200tr on lipopolysaccharide-stimulated dendritic (DCs). The ability each linked murine IgG2aFc, suppress allogeneic immune responses vitro (mixed leukocyte cultures) vivo (skin rejection) alone, combination, studied.CHO expressing inhibited suppression mixed reactions seen addition (CD200Fc) culture. In addition, prepared by fusing extracellular domain IgG2a Fc region, could block competitive fashion CD200Fc-mediated cytotoxic T lymphocyte induced reactions, tumor necrosis factor-alpha produced splenic cells, skin rejection vivo.Taken together our data support hypothesis variant antagonist CD200.