Urinary Peptide Patterns in Native Kidneys and Kidney Allografts

• DOI: 10.1097/tp.0b013e3181a66595
• Publication Date: 2009-06-17T07:21:51Z
• Authors: Yan Zhang; William S. Oetting; Stephen B. Harvey; Matthew D. Stone; Teresa Monkkonen; Arthur J. Matas; Fernando G. Cosio; Gary L. Nelsestuen
• Keywords: Beta-2 microglobulin; Nephrology

Background. The use of biopsies to determine kidney health after transplantation is an invasive procedure with some risk the patient. Consequently, a noninvasive test for transplanted would provide significant advantage over current clinical practice. Methods. Urines from donors before nephrectomy, pretransplant patients native disease, and posttransplant recipients were examined protein biomarkers diagnose or prognose disease. Proteins extracted by C4 reverse phase affinity analyzed matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Results. Urine individuals healthy kidneys showed few components other than two ubiquitous saposin B glycoisoforms. Patients disease lacked new in patterns: most common contained β-2 microglobulin (B2M, m/z=11,732) plus one more peaks at m/z=10,350, 9480, 4337, 4180. Pattern 2 but several degradation products α-1 antitrypsin. Other pathologic included urinary 1 (m/z=15,835), transthyretin (m/z=13,880), component m/z=13,350. Conclusions. acute rejection profiles that ranged those advanced range patterns may be useful analysis transplant without complications persons developing transplant.