Influence of Donor Age on Islet Isolation and Transplantation Outcome

• DOI: 10.1097/tp.0b013e31820385e6
• Publication Date: 2010-11-30T13:03:00Z
• Authors: Nadja Niclauss; Domenico Bosco; Philippe Morel; Sandrine Demuylde...; Coralie Brault; Laure Milliat-Gui...; Cyrille Colin; Geraldine Parnaud; Yannick D. Muller; Laurianne Giovannoni; Raphael Meier; Christian Toso; Lionel Badet; Pierre-Yves Benhamou; Thierry Berney
• Keywords: Adult; Blood Glucose; Male; MESH: Tissue Donors; Islets of Langerhans Transplantation; Hypoglycemic Agents/therapeutic use; MESH: Insulin; Islets of Langerhans; 03 medical and health sciences; MESH: Hypoglycemic Agents; info:eu-repo/classification/ddc/617; [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology; Humans; Hypoglycemic Agents; Insulin; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology; MESH: Treatment Outcome; Retrospective Studies; MESH: Age Factors; Diabetes Mellitus, Type 1/drug therapy/surgery; 0303 health sciences; MESH: Middle Aged; MESH: Humans; ddc:617; Blood Glucose/metabolism; MESH: Islets of Langerhans; Age Factors; MESH: Adult; MESH: Retrospective Studies; Middle Aged; MESH: Male; Tissue Donors; Islets of Langerhans Transplantation/physiology; Diabetes Mellitus, Type 1; Treatment Outcome; MESH: Islets of Langerhans Transplantation; Insulin/therapeutic use; Islets of Langerhans/physiology/surgery; MESH: Blood Glucose; Female; MESH: Diabetes Mellitus, Type 1; MESH: Female

It has been suggested that the age of human organ donors might influence islet isolation and transplantation outcome in a negative way due to a decrease of in vivo function in islets isolated from older donors.We retrospectively analyzed 332 islet isolations according to donor age. We determined isolation outcome by islet yields, transplantation rates, and [beta]-cell function in vitro. Transplanted patients were divided into two groups depending on donor age (n=25 and n=31 patients for <=45- and >45-year-old donors, respectively). We assessed islet graft function by C-peptide/glucose ratio, [beta] score, secretory units of islets in transplantation index, and insulin independence rate at 1, 6, and 12 months after transplantation.There was no difference in islet yields between the two groups (251,900+/-14,100 and 244,600+/-8400 islet equivalent for <=45- and >45-year-old donors, respectively). Transplantation rates and stimulation indices were similar in both groups as well. All islet graft function parameters were significantly higher at 1-month follow-up in patients who had received islets from younger donors. At 6-month follow-up after second or third injection and at 12-month follow-up, secretory units of islets in transplantation indices and C-peptide/glucose ratios were significantly higher in patients with donors aged 45 years or younger.These data suggest that, despite similar outcomes of the isolation procedure, islet graft function is significantly influenced by donor age. These results may have important consequences in the definition of pancreas allocation criteria.