Effectiveness of Apoptotic Factors Expressed on the Wounds of Patients With Stage III Pressure Ulcers

• DOI: 10.1097/won.0b013e318259c47e
• Publication Date: 2012-05-30T07:20:15Z
• Authors: Liping Jiang; En Zhang; Yeqin Yang; Chunyu Zhang; Xiaobing Fu; Jian Xiao
• Keywords: Fas ligand

PURPOSE: The purpose of this study was to explore the expression apoptotic factors in stage III pressure ulcers and explain their effects on wound healing. SUBJECTS AND SETTING: sample comprised 8 patients with ulcers, along 4 volunteers normal skin who were undergoing elective surgical procedures. METHODS: Eight patient tissue specimens from obtained hospitalized patients. Tissue volunteer during surgery. All 12 analyzed for presence apoptosis-related including bax/bcl-2 (B-cell lymphoma 2), Fas/fasL (Fas ligand), terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) as well immunohistochemical analysis Fas/FasL. RESULTS: Bax positive cells strongly expressed surrounding zone center Bcl-2 staining decreased contrarily (P < .01). Comparison, bax cell fas/fasL mRNA occurred rarely control group. mRNA-positive granules detected epidermal fibroblasts, demonstrating a progressive increase margin progressing toward bed. We also found an increased intensity TUNEL ulcer margin, compared tissue. CONCLUSION: These results suggest that molecular regulators apoptosis are involved Results differences distribution related play important role delayed healing characteristic these complex wounds.