Impact of neurite alignment on organelle motion

• DOI: 10.1098/rsif.2021.0617
• Publication Date: 2022-02-09T08:10:09Z
• Authors: Maria Mytiliniou; Joeri A. J. Wonde...; Thomas Schmidt; Doris Heinrich
• Keywords: Neurite; Organelle; Growth cone

Intracellular transport is pivotal for cell growth and survival. Malfunctions in this process have been associated with devastating neurodegenerative diseases, highlighting the need a deeper understanding of mechanisms involved. Here, we use an experimental methodology that leads neurites differentiated PC12 cells into either one two configurations: one-dimensional configuration, where align along lines, or two-dimensional adopt random orientation shape on flat substrate. We subsequently monitored motion functional organelles, lysosomes, inside neurites. Implementing time-resolved analysis mean-squared displacement, quantitatively characterized distinct modes lysosomes. Our results indicate neurite alignment gives rise to faster diffusive super-diffusive lysosomal than situation which are randomly oriented. After inducing lysosome swelling through osmotic challenge by sucrose, confirmed predicted slowdown mobility. Surprisingly, found swelling-induced mobility change affected each (sub-/super-)diffusive differently depended configuration findings imply intracellular significantly robustly dependent morphology, might part be controlled extracellular matrix.