Genetic polymorphism of Plasmodium falciparum msp-1, msp-2 and glurp vaccine candidate genes in pre-artemisinin era clinical isolates from Lakhimpur district in Assam, Northeast India

• DOI: 10.1099/acmi.0.000350
• Publication Date: 2022-04-25T12:16:45Z
• Authors: Vinayagam Sathish...; Tulika Nirmolia; Dibya Ranjan Bhat...; Saurav Jyoti Patgiri
• Keywords: Merozoite surface protein

Northeast India shares its international border with Southeast Asia and has a number of malaria endemic zones. Monitoring genetic diversity parasites is important in this area as drug resistance increasing form vicious cycle which one favours the development other. This retrospective study was done to evaluate patterns Plasmodium falciparum strains circulating North Lakhimpur Assam pre-artemisinin era compare findings current patterns.Genomic DNA extraction from archived blood spot samples collected 2006 malaria-positive cases district Assam, India. Three antigenic markers were studied - msp-1 (block-2), msp-2 (block-3) glurp RII region P. using nested PCR.Allelic examined 71 isolates high polymorphism observed. In msp-1, eight genotypes detected; K1 (single allele), MAD20 (six different alleles) RO33 allele) allelic families noted. Among genotypes, 22 distinct alleles observed out FC27 had six IC/3D7 16 alleles. glurp, nine obtained. Expected heterozygosity (HE) values three 0.72, 0.81 0.88, respectively. Multiplicity infection (MOI) noted 1.28, 1.84 1.04 for respectively.Results suggest level msp (block-2 block-3 msp-2) when chloroqunine primary used uncomplicated malaria. Comparison studies have revealed that these genes still region, complicating vaccine research.