Genetic characterization of Escherichia coli and Klebsiella spp. from Humans and Poultry in Nigeria

• DOI: 10.1099/acmi.0.000509.v2
• Publication Date: 2023-03-03T12:20:42Z
• Authors: Christiana Jesumi...; Adriana CABAL-ROSEL; Franz ALLERBERGER; Burkhard SPRINGER; Werner RUPPITSCH
• Keywords: Klebsiella; Multilocus sequence typing

The emergence of antibiotic resistance in food producing animals is major public health significance arising from the risk these bacteria entering chain. In this study, genetic characteristics resistant Escherichia coli and Klebsiella spp. isolates humans poultry Edo state, Nigeria were investigated. April 2017, 45 46 obtained urine, clinical wounds, nasal chicken fecal samples. Isolates recovered identified as previously described. Species identification was achieved by matrix-assisted laser desorption ionization-time flight (MALDI-TOF) mass spectrometry ribosomal multilocus sequence typing. Antimicrobial susceptibility testing carried out using Kirby-Bauer method for 12 antibiotics. Double disc synergy test used to screen ESBL production. Whole genome sequencing performed strain characterization isolates. Thirteen yielded positive results phenotypic harbored genes. Out E. isolates, 21 human 13 at least one tested Four genes revealed when applying double tests. include bla CTX-M-15 SHV-28 . based cgMLST a close relatedness among An integrated “One Health” surveillance system required monitor transmission antimicrobial Nigeria.