Detection of clinically relevant antibiotic resistant bacteria in shared fomites, waste water, and municipal solid wastes disposed in vicinity of residential areas of Nigerian Urban City

• DOI: 10.1099/acmi.0.000641.v3
• Publication Date: 2023-11-24T15:30:49Z
• Authors: Ibrahim Yusuf; Zainab Damji Muha...; Binta Muhammad Amin; Maryam Danladi Sh...; Nafisatu Hamza; Hajara Dauda Isah; Nasir Bako Abdullahi; Patience James Ene; Sameera Salisu Sh...; Nasir Doguwa; Shamsudeen Lekan ...; Maryam Adamu Muha...
• Keywords: Acinetobacter baumannii

SStudies investigating environmental hotspots of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Nigeria are limited. This study was designed to assess various sources commonly touched surfaces as potential carriers ARB ARGs with implications for public health. A total 392 samples, including sewage (36), sludge diapers (20), plastics water sachet polythene bags food wastes soil beneath dump sites frequently like restroom floors (80), corridors (24), door handles (56), room walls (60), were collected screened the presence resistant carrying such blaKPC, blaNDM-1, blaCMY-2, blaIMP, blaOXA66, MecA. Additionally, we employed standard techniques detect methicillin-resistant Staphylococcus aureus (MRSA) extended-spectrum β-lactamase-producing Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii. We also evaluated effectiveness routine disinfection procedures eliminating from floors. Our findings revealed that sewage, sludge, diapers, wastes, contaminated highly moderately strains E. K. P. MRSA. Notably, identified two variants blaOXA51-like gene (blaOXA-66 & blaOXA-180) A. baumannii isolated these sources. Furthermore, detected seven ESBL-K. five ESBL-A. baumannii, ESBL-E. one ESBL-P. all or more (blaKPC, blaCMY-2), isolates recovered floors, plastics. It is worth noting persisted on even after procedures. In conclusion, this highlights indiscriminately discarded residential areas shared among individuals heavily colonized by significant health importance.