DETECTION AND ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM SELECTED POULTRY FARMS IN DAR ES SALAAM, TANZANIA.

• DOI: 10.1099/acmi.0.000879.v3
• Publication Date: 2025-02-28T14:23:41Z
• Authors: Saxon Joseph Mwam...; Adelard B. Mtenga; Augustino A. Chen...; Abubakar S. Hoza
• Keywords: Dar es salaam; Poultry farming

Introduction Salmonella, one of the most frequent foodborne bacteria, is linked to 155,000 annual mortality and 93.8 million illnesses. Rampant use antibiotic agents combat Salmonellosis in poultry has contributed emergence resistance against commonly used antibiotics. Methodology A cross-section study was conducted between January June 2023. Purposive sampling select farms, faecal droppings were collected determine occurrence multidrug-resistant (MDR) Salmonella spp. Samples examined for presence by standard microbiological techniques. Conventional biochemical tests molecular methods such as PCR partial DNA sequencing identification isolates. Antimicrobial susceptibility testing performed identify isolates resistant seven classes Results The overall spp isolated from selected farms 6.04% (n=48). Of PCR-confirmed isolates, 64.3% (n=18) more than two antibiotics hence considered MDR. highest observed with ampicillin (92.9%), followed tetracycline (69%), ciprofloxacin (42.9%), sulfonamide gentamicin (35.7%), azithromycin (28.9%). All susceptible chloramphenicol (100%). Twenty-eight (28) sequenced only sixteen (16) sequences met criteria phylogenetic analysis. Sequences clustered together enterica sub accession number EU348369 which chickens, pigs, foods. Conclusion high level found this could be attributed unwarranted To limit farming at farm level, immediate action needed including advocating prudent production systems strengthening extension services farmers biosecurity.