Genotypic and Phenotypic screening of agriculturally based antimicrobial resistant E. coli.

• DOI: 10.1099/acmi.0.000988.v2
• Publication Date: 2025-04-08T11:23:49Z
• Authors: Hayley Marshall; Colman O'Cathail; Katie Kelly; Jessica Hardwick; Sabine Tötemeyer; Adam Mark Blanchard
• Keywords: Phenotypic screening

Antimicrobial resistance is undoubtedly one of the most significant threats to human health. Whilst finding alternative antimicrobials proves valuable, understanding how spread remains a necessity. The link between antimicrobial in agriculture and effect on medicine unclear, however contribution dissemination should not be overlooked. Escherichia coli (E. coli) are particular concern for health as common Gram-negative pathogen often used representative indicator resistance. Sheep hold unique role agriculture, large numbers transported multiple farms make use seasonal grazing. We present robust analysis multi-antimicrobial profiles, using short read metagenomics long sequencing, identify plasmid borne well chromosomal mutational resistances. these data highlighted putative plasmids 85% isolates with genes against commonly antimicrobials, such streptomycin, tetracycline, sulphonamide, licosamide, betalactam, macrolide, kanamycin, fosfomycin, chloramphenicaol, cephalosporin aminoglycoside.