Serinibacter tropicus sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove, and emended description of the genus Serinibacter

• DOI: 10.1099/ijs.0.000068
• Publication Date: 2015-01-21T04:58:11Z
• Authors: Moriyuki Hamada; Chiyo Shibata; Arif Nurkanto; Shanti Ratnakomala; Puspita Lisdiyanti; Tomohiko Tamura; Ken-ichiro Suzuki
• Keywords: DNA, Bacterial; Islands; 0301 basic medicine; Base Composition; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Vitamin K 2; Peptidoglycan; Sequence Analysis, DNA; 03 medical and health sciences; Indonesia; RNA, Ribosomal, 16S; Actinomycetales; Rhizosphere; Avicennia; Phylogeny

A novel Gram-stain-positive actinobacterium, designated PS-14-7T, was isolated from the rhizosphere of a mangrove on Pramuka Island, Indonesia, and its taxonomic position was investigated using a polyphasic approach. The peptidoglycan type of strain PS-14-7T was A4α and lysine was the diagnostic diamino acid of the peptidoglycan. The predominant menaquinone was MK-8(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The DNA G+C content was 72.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain PS-14-7T was closely related to

Serinibacter salmoneus

Kis4-28T (99.6 %). However, DNA–DNA hybridization and phenotypic characteristics revealed that strain PS-14-7T differed from

Serinibacter salmoneus

. Therefore, strain PS-14-7T represents a novel species of the genus

Serinibacter

, for which the name Serinibacter tropicus sp. nov. is proposed. The type strain is PS-14-7T ( = NBRC 110108T = InaCC A 515T). An emended description of the genus

Serinibacter

is also proposed.