Parabacteroides faecis sp. nov., isolated from human faeces

• DOI: 10.1099/ijs.0.000109
• Publication Date: 2015-02-10T04:08:36Z
• Authors: Mitsuo Sakamoto; Yoshiki Tanaka; Yoshimi Benno; Moriya Ohkuma
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Composition; 0303 health sciences; Bacteroidetes; Fatty Acids; Molecular Sequence Data; Vitamin K 2; Chaperonin 60; Sequence Analysis, DNA; Bacterial Typing Techniques; 3. Good health; Feces; 03 medical and health sciences; Bacterial Proteins; 13. Climate action; RNA, Ribosomal, 16S; Bile; Humans; Phylogeny

A bacterial strain, designated 157T, isolated from human faeces was characterized by using a polyphasic taxonomic approach, which included analysis of physiological and biochemical features, cellular fatty acid profiles, menaquinone profiles and its phylogenetic position, based on 16S rRNA gene sequence analysis. The strain was obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. The isolate was able to grown on medium containing 20 % (w/v) bile. 16S rRNA gene sequence analysis showed that the strain was a member of the genusParabacteroides. Strain 157Twas closely related toParabacteroides gordoniiJCM 15724T(96 % sequence similarity). The results ofhsp60gene sequence analysis indicated that strain 157Twas different fromP. gordoniiJCM 15724T, with ahsp60gene sequence similarity of 96.1 %. The major cellular fatty acids of strain 157Twere anteiso-C15 : 0, iso-C17 : 03-OH, C18 : 1ω9cand anteiso-C17 : 03-OH. The major menaquinone of the isolate was MK-9. The DNA G+C content of strain 157Twas 41.8 mol%. On the basis of these data, strain 157Trepresents a novel species of the genusParabacteroides, for which the nameParabacteroides faecissp. nov. is proposed; the type strain is 157T( = JCM 18682T = CCUG 66681T).