Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil

• DOI: 10.1099/ijs.0.000198
• Publication Date: 2015-03-21T03:12:00Z
• Authors: Kaibiao Wang; Lei Zhang; Yang Yang; Yuanyuan Pan; Lin Meng; Henan Liu; Shan Hong; Haipeng Huang; Juquan Jiang
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Composition; China; Salinity; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Vitamin K 2; Peptidoglycan; Sequence Analysis, DNA; Hydrogen-Ion Concentration; Diaminopimelic Acid; 6. Clean water; Soil; 03 medical and health sciences; RNA, Ribosomal, 16S; Halobacillus; Phospholipids; Phylogeny; Soil Microbiology

A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3–15 % (w/v) (optimum, 8 %, w/v), at pH 7.0–9.0 (optimum, pH 8.0) and at 4–60 °C (optimum, 30 °C). It containedmeso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genusHalobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40Tand the type strains of related species of the genusHalobacillusranged from 98.8 % (Halobacillus alkaliphilusFP5T) to 97.1 % (Halobacillus kuroshimensisIS-Hb7T). DNA–DNA hybridization relatedness values between strain NEAU-ST10-40TandH. alkaliphilusDSM 18525T,Halobacillus campisalisKCTC 13144T,Halobacillus yeomjeoniDSM 17110T,Halobacillus halophilusDSM 2266T,Halobacillus litoralisDSM 10405T,Halobacillus dabanensisDSM 18199T,Halobacillus salinusDSM 18897T,Halobacillus naozhouensisDSM 21183T,Halobacillus trueperiDSM 10404TandHalobacillus salsuginisDSM 21185Twere from 43 ± 1 to 19 ± 1 % (mean ± sd). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C15:0, anteiso-C17:0and iso-C16:0, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40Tis considered to represent a novel species, for which the nameHalobacillus andaensissp. nov. is proposed. The type strain is NEAU-ST10-40T( = CGMCC 1.12153T = DSM 25866T).