Camelimonas fluminis sp. nov., a cyhalothrin-degrading bacterium isolated from river water

• DOI: 10.1099/ijs.0.000384
• Publication Date: 2015-06-25T14:12:18Z
• Authors: Long Zhang; Man Song; Qin Cao; Shang Wu; Yan Zhao; Jun-Wei Huang; Kai Chen; Shun-Peng Li; Zhen-Yuan Xia; Jian-Dong Jiang
• Keywords: DNA, Bacterial; 0301 basic medicine; Base Composition; China; 0303 health sciences; Spermidine; Ubiquinone; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Sequence Analysis, DNA; 16. Peace & justice; 6. Clean water; Bacterial Typing Techniques; 03 medical and health sciences; Rivers; Beijerinckiaceae; RNA, Ribosomal, 16S; Nitriles; Pyrethrins; Phospholipids; Phylogeny

An aerobic, Gram-stain-negative, short rod-shaped, non-spore-forming, cyhalothrin-degrading bacterial strain, XZ2T, was isolated from the surface water of Hanjiang River in Wuhan, China. Strain XZ2T grew optimally at pH 6.0, 30 °C and in the absence of NaCl. The G+C content of the total DNA was 64.1 mol%. The 16S rRNA gene sequence of strain XZ2T showed the highest similarity to that of Camelimonas lactis M 2040T (99.1 %), followed by Camelimonas abortus UK34/07-5T (95.9 %) and Chelatococcus daeguensis K106T (95.3 %). The major cellular fatty acids of strain XZ2T were C19 : 0 cyclo ω8c (63.1 %), C16 : 0 (15.0 %) and C18 : 1ω7c/C18 : 1ω6c (summed feature 8; 8.9 %). C18 : 0 3-OH was also detected as the major hydroxylated fatty acid. The respiratory quinone was ubiquinone Q-10. The polar lipid profile included the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylglycerol and two unidentified aminolipids. The predominant compound in the polyamine pattern was spermidine. These chemotaxonomic data supported the affiliation of strain XZ2T to the genus Camelimonas. The DNA–DNA hybridization value between strain XZ2T and Camelimonas lactis M 2040T was 43.5 ± 0.6 %. DNA–DNA hybridization data as well as biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiations between strain XZ2T and Camelimonas lactis M 2040T. Therefore, strain XZ2T represents a novel species of the genus Camelimonas, for which the name Camelimonas fluminis sp. nov. is proposed. The type strain is XZ2T ( = KCTC 42282T = ACCC 19738T).