Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans

• DOI: 10.1099/ijs.0.001230-0
• Publication Date: 2009-01-06T19:38:52Z
• Authors: A. Nemec; M. Musilek; M. Maixnerova; T. De Baere; T. J. K. van der ...; M. Vaneechoutte; L. Dijkshoorn
• Keywords: DNA, Bacterial; Restriction Mapping; HUMAN CLINICAL SPECIMENS; DNA, Ribosomal; Hemolysis; 03 medical and health sciences; BAUMANNII; Species Specificity; RNA, Ribosomal, 16S; Medicine and Health Sciences; Environmental Microbiology; Animals; Humans; Phylogeny; 0303 health sciences; IDENTIFICATION; Acinetobacter; STRAINS; RECOGNITION; Nucleic Acid Hybridization; Genes, rRNA; DNA-Directed RNA Polymerases; Sequence Analysis, DNA; TRANSFORMATION; Bacterial Typing Techniques; Culture Media; Phenotype; CALCOACETICUS; GENUS ACINETOBACTER; Polymorphism, Restriction Fragment Length; Acinetobacter Infections

The taxonomic status of 24 haemolytic, non-glucose acidifying Acinetobacter strains that did not belong to any previously described species was investigated by means a polyphasic approach. Using AFLP fingerprinting, amplified rDNA restriction analysis and phenotypic characterization, the were classified into two phenetically coherent groups (comprising 15 9 strains) distinct from each other all known species. Confirmation these formed separate lineages within genus obtained comparative partial sequences gene encoding β-subunit RNA polymerase in also 16S rRNA sequence representative strains. Previously published DNA–DNA reassociation data for some used supported rank both groups, which names beijerinckii sp. nov. gyllenbergii are proposed. A. originated human animal specimens various environmental sources, whereas those isolated exclusively clinical specimens. characteristics most useful differentiation comprise haemolytic inability grow on l-arginine ability azelate. type strain is NIPH 838T (=LUH 4759T=CCUG 51249T=CCM 7266T=58aT) 2150T (=RUH 422T=CCUG 51248T=CCM 7267T=1271T).