Brucella inopinata sp. nov., isolated from a breast implant infection

• DOI: 10.1099/ijs.0.011148-0
• Publication Date: 2009-11-03T22:56:16Z
• Authors: Holger C. Scholz; Karsten Nöckler; Cornelia Göllner; Peter Bahn; Gilles Vergnaud; Herbert Tomaso; Sascha Al Dahouk; Peter Kämpfer; Axel Cloeckaert; Marianne Maquart; Michel S. Zygmunt; Adrian M. Whatmore; Martin Pfeffer; Birgit Huber; Hans-Jürgen Busse; Barun Kumar De
• Keywords: Multilocus sequence typing; Biovar; Brucellaceae

A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1(T)) was isolated recently from a breast implant infection of 71-year-old female patient with clinical signs brucellosis. Affiliation strain BO1(T) to the genus Brucella confirmed by means polyamine pattern, polar lipid profile, fatty acid quinone system, DNA-DNA hybridization studies and insertion sequence 711 (IS711)-specific PCR. Strain harboured four five copies Brucella-specific element IS 711, displaying unique banding exhibited 16S rRNA gene also grouped separately in multilocus typing analysis. reacted M-monospecific antiserum. Incomplete lysis detected bacteriophages Tb (Tbilisi), F1 F25. Biochemical profiling revealed high degree enzymic activity metabolic capabilities. In VNTR (variable-number tandem-repeat) analysis, showed very distinctive profile clustered other 'exotic' strains, including strains marine mammals, microti, suis biovar 5 neotomae. Comparative omp2a omp2b analysis most divergent omp2 sequences identified date for strain. The recA differed seven nucleotides consensus sequence. Using species-specific multiplex PCR assay, displayed pattern not observed species. From phenotypic molecular it became evident that BO1( T) clearly different all species, therefore represents novel species within Brucella. Because its unexpected isolation, name inopinata type (=BCCN 09-01(T)=CPAM 6436(T)) is proposed.