Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces

• DOI: 10.1099/ijs.0.024521-0
• Publication Date: 2010-12-11T02:56:17Z
• Authors: Hidetoshi Morita; Akiyo Nakano; Hiromi Onoda; Hidehiro Toh; Kenshiro Oshima; Hideto Takami; Masaru Murakami; Shinji Fukuda; Tatsuya Takizawa; Tomomi Kuwahara; Hiroshi Ohno; Soichi Tanabe; Masahira Hattori
• Keywords: DNA, Bacterial; Male; 0301 basic medicine; Base Composition; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Infant; beta-Galactosidase; 6. Clean water; 3. Good health; Feces; 03 medical and health sciences; Bacterial Proteins; RNA, Ribosomal, 16S; Cellulases; Humans; Bifidobacterium; Phylogeny

Strains HM2-1 and HM2-2(T) were isolated from the faeces of a healthy infant characterized by determining their phenotypic biochemical features phylogenetic positions based on partial 16S rRNA gene sequence analysis. They Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, catalase-negative non-motile rods. did not grow at 15 or 45 °C in anaerobic bacterial culture medium, DNA G+C content was range 56-59 mol%. In enzyme activity tests, strains positive for α/β-galactosidases α/β-glucosidases but negative β-glucuronidase cystine arylamidase. An analysis cell-wall composition revealed presence glutamic acid, alanine lysine. The fructose-6-phosphate phosphoketolase shows that isolates are members genus Bifidobacterium. These two belong to same species Strain found be related Bifidobacterium catenulatum JCM 1194(T) (97.4 % identity: 1480/1520 bp), pseudocatenulatum 1200(T) (97.2 %: 1472/1514 dentium ATCC 27534(T) (96.7 1459/1509 bp) angulatum 27535(T) (96.5 1462/1515 bp). predominant cellular fatty acids 16 : 0 18 1ω9c, with proportions greater than total. Phylogenetic analyses involving characterization, DNA-DNA hybridization sequencing proves represent novel Bifidobacterium, which name kashiwanohense sp. nov. is proposed. type strain ( = 15439(T) DSM 21854(T)).