Pyrococcus yayanosii sp. nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent

• DOI: 10.1099/ijs.0.024653-0
• Publication Date: 2011-01-15T03:00:41Z
• Authors: Jean-Louis Birrien; Xiang Zeng; Mohamed Jebbar; Marie-Anne Cambon...; Joël Quérellou; Philippe Oger; Nadège Bienvenu; Xiang Xiao; Daniel Prieur
• Keywords: Obligate anaerobe; Strain (injury); Yeast extract; Hyperthermophile; Hydrostatic pressure

An obligate piezophilic anaerobic hyperthermophilic archaeon, designated strain CH1(T), was isolated from a hydrothermal vent site named 'Ashadze', which is located on the Mid-Atlantic Ridge at depth of 4100 m. Enrichment and isolation were carried out 95 °C under hydrostatic pressure 42 MPa. Cells CH1(T) highly motile irregular cocci with diameter ~1-1.5 µm. Growth recorded 80-108 (optimum 98 °C) pressures 20-120 MPa 52 MPa). No growth observed atmospheric 60-110 °C. pH 6.0-9.5 7.5-8.0) in 2.5-5.5% (w/v) NaCl 3.5%). Strain strictly grew complex proteinaceous substrates, such as yeast extract, Peptone, casein, well sucrose, starch, chitin, pyruvate, acetate glycerol without electron acceptors. The G+C content genomic DNA 49.0±0.5 mol%. Analysis 16S rRNA gene sequences revealed that belongs to genus Pyrococcus. Based its physiological properties similarity levels between ribosomal proteins, represents novel species, for name Pyrococcus yayanosii sp. nov. proposed. type (=JCM 16557). This also available by request Souchothèque de Bretagne (catalogue LMBE) culture collection (collection no. 3310).