Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China

• DOI: 10.1099/ijs.0.02840-0
• Publication Date: 2004-11-15T23:14:12Z
• Authors: Jun-Lian Gao; Sarah Lea Turner; Feng Ling Kan; En Tao Wang; Zhi Yuan Tan; Yu Hui Qiu; Jun Gu; Zewdu Terefework; J. Peter W. Young; Kristina Lindström; Wen Xin Chen
• Keywords: Mesorhizobium; Biovar

Ninety-five rhizobial strains isolated from Astragalus adsurgens growing in the northern regions of China were classified into three main groups, candidate species I, II and III, based on a polyphasic approach. Comparative analysis full-length 16S rRNA gene sequences representative showed that I Mesorhizobium , while which consisted non-nodulating strains, was closely related to Agrobacterium tumefaciens . The phylogenetic relationships some also confirmed by sequencing glnA genes, used as an alternative chromosomal marker. DNA–DNA relatedness between 11·3 47·1 % among type defined species. Candidate III had DNA 4·3 25·2 with rubi Two novel are proposed accommodate II, septentrionale sp. nov. (type strain, SDW014 T =CCBAU 11014 =HAMBI 2582 ) temperatum SDW018 11018 2583 ), respectively. At least two distinct nodA identified strains. numerically dominant sequence most similar tianshanense strain belonging well other, yet, undefined genome types. Host range studies indicate different correlate host ranges. Further comparative needed clarify taxonomic identity III.