Solirubrobacter ginsenosidimutans sp. nov., isolated from soil of a ginseng field

• DOI: 10.1099/ijs.0.028431-0
• Publication Date: 2010-12-11T02:56:17Z
• Authors: Dong-Shan An; Liang Wang; Minseok S. Kim; Heon-Meen Bae; Sung-Taik Lee; Wan-Taek Im
• Keywords: Strain (injury); genomic DNA

A Gram-reaction-positive, non-motile, non-spore-forming, aerobic rod, designated BXN5-15(T), was isolated from the soil of a ginseng field on Baekdu Mountain in China. Strain BXN5-15(T) grew optimally at 30 °C and pH 6.0-7.0 absence NaCl R2A agar. displayed β-glucosidase activity, which allowed it to transform ginsenoside Rb(1) (one dominant active components ginseng) via Rd minor F(2). On basis 16S rRNA gene sequence analysis, strain shown belong genus Solirubrobacter. The closest phylogenetic relatives were Solirubrobacter soli Gsoil 355(T) (98.4 % similarity) pauli B33D1(T) (96.4 %). Lower similarities (<96.0 %) found with all other recognized members order Solirubrobacterales. predominant quinone MK-7(H(4)). major fatty acids (>10 C(18 : 1)ω9c, iso-C(16 0) 3)ω6,9,12c. G+C content genomic DNA 70.6 mol%. DNA-DNA relatedness between S. KCTC 12628(T) 23.3 %. genotypic, phenotypic chemotaxonomic data, represents novel species within Solirubrobacter, for name ginsenosidimutans sp. nov. is proposed. type ( = KACC 20671(T) LMG 24459(T)).