Paenibacillus oceanisediminis sp. nov. isolated from marine sediment

• DOI: 10.1099/ijs.0.037085-0
• Publication Date: 2012-03-31T03:47:47Z
• Authors: Jina Lee; Na-Ri Shin; Mi-Ja Jung; Seong Woon Roh; Min-Soo Kim; Jung-Sook Lee; Keun Chul Lee; Young-Ok Kim; Jin-Woo Bae
• Keywords: DNA, Bacterial; Base Composition; Geologic Sediments; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Vitamin K 2; Aquaculture; Sequence Analysis, DNA; 6. Clean water; Bacterial Typing Techniques; 03 medical and health sciences; RNA, Ribosomal, 16S; Republic of Korea; Seawater; Paenibacillus; Phylogeny

A Gram-stain-negative, non-motile, aerobic, endospore forming and rod-shaped bacterium, designated strain L10T, was isolated from marine sediment collected from the South Korean coast. The organism grew optimally under conditions of 30 °C, 1 % (w/v) NaCl and pH 6.0. It was oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L10T was associated with the genus

Paenibacillus

and most closely related to

Paenibacillus barcinonensis

BP-23T (98.2 % similarity). The major fatty acids of strain L10T were iso-C14 : 0, anteiso-C15 : 0 and iso-C16 : 0. The cell-wall peptidoglycan was the A1γ type, and the predominant isoprenoid quinone was menaquinone-7. Strain L10T contained two unidentified lipids, an unidentified amino-phospholipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The G+C content of the genomic DNA was 44 mol% and the DNA–DNA hybridization values with closely related strains were below 14±2 %. Based on phenotypic, genotypic, and phylogenetic data, strain L10T should be classified as a novel species within the genus

Paenibacillus

. The name Paenibacillus oceanisediminis sp. nov. is proposed. The type strain is L10T ( = KACC 16203T = JCM 17814T).