Phyllobacterium endophyticum sp. nov., isolated from nodules of Phaseolus vulgaris

• DOI: 10.1099/ijs.0.038497-0
• Publication Date: 2012-05-20T13:00:30Z
• Authors: José-David Flores...; Lorena Carro; Encarna Velázquez; Ángel Valverde; Eugenia Cerda-Cas...; Paula García-Fraile; Raúl Rivas
• Keywords: DNA, Bacterial; Phaseolus; Base Composition; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Quinones; Nucleic Acid Hybridization; Phyllobacteriaceae; Sequence Analysis, DNA; Bacterial Typing Techniques; 03 medical and health sciences; Genes, Bacterial; Spain; RNA, Ribosomal, 16S; Root Nodules, Plant; Phylogeny

A strain, PEPV15T, was isolated from a nodule onPhaseolus vulgarisgrown in soil in northern Spain. Phylogenetic analyses of 16S rRNA andatpDgenes showed that this strain belongs to the genusPhyllobacterium.The most closely related species were, in both cases,Phyllobacterium brassicacearum,Phyllobacterium bourgognenseandPhyllobacterium trifolii, the type strains of which gave sequence similarities of 98.9, 98.6 and 98.4 %, respectively, in the 16S rRNA gene and 88.1, 87.5 and 88.7 %, respectively, in theatpDgene. PEPV15Tcontained Q-10 as the major quinone (88 %) and low amounts of Q-9 (12 %). It differed from its closest relatives in its growth in diverse culture conditions and in the assimilation of several carbon sources. The strain was not able to produce nodules inPhaseolus vulgaris.The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genusPhyllobacteriumfor which the namePhyllobacterium endophyticumsp. nov. is proposed; the type strain is PEPV15T( = LMG 26470T = CECT 7949T). An emended description of the genusPhyllobacteriumis also provided.