Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments

• DOI: 10.1099/ijs.0.040568-0
• Publication Date: 2012-06-17T21:34:07Z
• Authors: Hisako Hirayama; Hiroyuki Fuse; Mariko Abe; Masayuki Miyazaki; Takamichi Nakamura; Takuro Nunoura; Yasuo Furushima; Hiroyuki Yamamoto; Ken Takai
• Keywords: Methanotroph; Methane Monooxygenase

Two aerobic methane-oxidizing bacterial strains were isolated from distinct marine environments in Japan. Strains IT-4(T) and T2-1 Gram-stain-negative, aerobic, motile, plump short rods or oval-shaped bacteria with a single polar flagellum type I intracytoplasmic membranes. They obligate methanotrophs that grew only on methane methanol. Each strain possessed the particulate monooxygenase (pMMO). The ribulose monophosphate pathway was operative for carbon assimilation. best at 37 °C, did not grow 45 °C. NaCl required growth within concentration range of 1-8 % (w/v). major phospholipid fatty acids C16 : 0, 1ω7c, 1ω5t. isoprenoid quinone MQ-8. DNA G+C content 50.9-51.7 mol%. 16S rRNA gene sequences showed 99.4 similarity to each other, DNA-DNA hybridization analysis indicated representatives same species. highly similar some environmental (94.0-97.7 similarity), but show similarities more than 94 members other related genera, such as Methylomicrobium, Methylobacter, Methylomonas Methylosarcina. Phylogenies based deduced partial PmoA sequences, physiological chemotaxonomic characteristics revealed represent novel species new genus family Methylococcaceae, which name Methylomarinum vadi gen. nov., sp. nov. is proposed. ( = JCM 13665(T) DSM 18976(T)).